Akademik Veri Yönetim Sistemi
2022 In Vitro Biology Meeting , California, Amerika Birleşik Devletleri, 4 - 07 Haziran 2022, cilt.58, ss.522
Cotton is among the most valuable crops in the world with a considerable effect on the economy due to its fiber manufacturing quality. Bt cotton plant is a major GMO crop containing genes encoding cry endotoxin group for insect-resistant. The aim of this study was to transfer the hybrid SN19 (cry1Ba- domain I–III and cry1Ia-domain II) and GUS reporter genes under the control of 35S promoter to cotton. First, embryonic axis, embryonic hypocotyl, and plumule explants were cultured in vitro for optimization of shoot regeneration. Reproducible in vitro plant regeneration was obtained from the embryonic axis. For gene transformation, embryo axis explants from mature seeds were inoculated with A. tumefaciensstrain GV2260 or EHA105 carrying the binary plasmid p35S-GUS-INT. After histochemical GUS, PCR and RT-PCR analysis, the transformation efficiency was recorded with varying GUS expression levels. In the second stage, to obtain insect-resistant and herbicide-tolerant plants the SN19 and bar genes were cloned into the T-DNA region of the pTF101.1 binary transformation plasmid and transferred successfully into cotton using embriyo axis explants. Putative transgenic cotton plants were primarily selected with phosphinothricin (PPT) thereafter both hybrid SN19 and bar genes confirmed by PCR analysis. Leaf bioassay with Lepidopteran Spodoptera exigua larvae showed 100% resistance.