In vitro Evaluation of Short-term Preserved Stallion Semen in Different Doses and Temperatures

TIRPAN M. B., AKÇAY E., ÇEBİ ŞEN Ç., Aksen U., Yildirim S., Tekin N.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.21, sa.4, ss.539-544, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 4
  • Basım Tarihi: 2015
  • Doi Numarası: 10.9775/kvfd.2014.12893
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.539-544
  • Anahtar Kelimeler: Stallion semen, Doses, Temperature, Short-term preservation, In vitro evaluation of semen, EQUINE SPERMATOZOAL MOTILITY, SEMINAL PLASMA, POSTTHAW MOTILITY, CRYOPRESERVATION, DILUTION, REMOVAL, CENTRIFUGATION, VIABILITY, STORAGE
  • Ankara Üniversitesi Adresli: Evet

Özet

This study was conducted to evaluate the effects of different temperatures and doses on stallion sperm survival after 24 h and 48 h short-term storage. Ejaculates from four warm-blooded stallions were used in this study. Ejaculates were collected by an open-ended artificial vagina which separates fractions of semen into sterile plastic cups. Only the second and third fractions (spermatozoa-rich fractions) were used to obtain spermatozoa for the experiment. Semen was diluted with INRA 96 extender to final concentrations of 50, 100, 200 and 400x10(6)sp/ml. Diluted semen were stored at different temperatures (2, 4 or 8 degrees C) for 24-48 h. After 24 h and 48 h cooled storage, spermatozoon motility, abnormal spermatozoon ratio and membrane integrity were evaluated. According to results the highest motility values (52.5% and 55%) were determined at 50 and 100 x10(6)sp/ml concentration at 4 degrees C after 24 h cooled storage. After 48 h of storage, the highest motility was obtained from semen stored at 8 degrees C. Abnormal spermatozoon and membrane integrity values were not significantly different after cooled storage. Consequently, it was determined that semen with 50 and 100 million spermatozoa/ml concentration stored at 4 degrees C for 24 h and semen with 50, 100, 200, 400 million spermatozoa/ml concentration stored at 8 degrees C for 48 h gave better results with respect to motility than the others.