In vitro Evaluation of Short-term Preserved Stallion Semen in Different Doses and Temperatures
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.21, sa.4, ss.539-544, 2015 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 21 Sayı: 4
- Basım Tarihi: 2015
- Doi Numarası: 10.9775/kvfd.2014.12893
- Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.539-544
- Anahtar Kelimeler: Stallion semen, Doses, Temperature, Short-term preservation, In vitro evaluation of semen, EQUINE SPERMATOZOAL MOTILITY, SEMINAL PLASMA, POSTTHAW MOTILITY, CRYOPRESERVATION, DILUTION, REMOVAL, CENTRIFUGATION, VIABILITY, STORAGE
- Ankara Üniversitesi Adresli: Evet
Özet
This study was conducted to evaluate the effects of different temperatures and doses on stallion sperm survival after 24 h and 48 h short-term storage. Ejaculates from four warm-blooded stallions were used in this study. Ejaculates were collected by an open-ended artificial vagina which separates fractions of semen into sterile plastic cups. Only the second and third fractions (spermatozoa-rich fractions) were used to obtain spermatozoa for the experiment. Semen was diluted with INRA 96 extender to final concentrations of 50, 100, 200 and 400x10(6)sp/ml. Diluted semen were stored at different temperatures (2, 4 or 8 degrees C) for 24-48 h. After 24 h and 48 h cooled storage, spermatozoon motility, abnormal spermatozoon ratio and membrane integrity were evaluated. According to results the highest motility values (52.5% and 55%) were determined at 50 and 100 x10(6)sp/ml concentration at 4 degrees C after 24 h cooled storage. After 48 h of storage, the highest motility was obtained from semen stored at 8 degrees C. Abnormal spermatozoon and membrane integrity values were not significantly different after cooled storage. Consequently, it was determined that semen with 50 and 100 million spermatozoa/ml concentration stored at 4 degrees C for 24 h and semen with 50, 100, 200, 400 million spermatozoa/ml concentration stored at 8 degrees C for 48 h gave better results with respect to motility than the others.