Detection and quantification of ATP in human blood serum

Akdeniz A., ÇAĞLAYAN M. G., Polivina I., Anzenbacher P.

ORGANIC & BIOMOLECULAR CHEMISTRY, cilt.14, sa.31, ss.7459-7462, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 31
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1039/c6ob01378c
  • Dergi Adı: ORGANIC & BIOMOLECULAR CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.7459-7462
  • Ankara Üniversitesi Adresli: Evet

Özet

Two fluorometric sensors based on the tri-serine tri-lactone scaffold and thiourea or sulfonamide moieties serving as hydrogen bond donors allowing for anion binding are described. The sensor utilizing thiourea as a recognition moiety shows fluorescence enhancement while the sensor with sulfonamide shows quenching upon addition of phosphates. Sensor arrays composed of two sensors are able to discriminate structurally similar organic phosphates in the presence of interferents in human blood serum. The quantitative analysis of ATP in human blood serum shows high accuracy (the root mean square error of prediction, 1.65%) without requiring any sample pretreatment.