The genome-wide characterization of WOX gene family in Phaseolus vulgaris L. during salt stress

Akbulut S. E., Okay A., Aksoy T., ARAS E. S., BÜYÜK İ.

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, cilt.28, sa.6, ss.1297-1309, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 6
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s12298-022-01208-1
  • Dergi Adı: PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.1297-1309
  • Anahtar Kelimeler: WOX gene family, Transcription factor, Bioinformatics, qRT-PCR, RNAseq, TRANSCRIPTION FACTORS, ORGAN DEVELOPMENT, IDENTIFICATION, EXPRESSION, TOLERANCE, ARABIDOPSIS, MAIZE, RICE, TIME, PCR
  • Ankara Üniversitesi Adresli: Evet

Özet

The WUSCHEL-Related Homeobox (WOX) family is a type of homeobox transcription factor superfamily and its members perform many functions ranging from plant embryonic growth to organ formation in plants. Although the WOX proteins have been identified and characterized in many plant species, genome-wide identification and characterization of WOX proteins in the Phaseolus vulgaris genome has been performed for the first time in this study. Accordingly, 18 WOX proteins were identified using bioinformatics tools and biochemical/physicochemical properties of these proteins were investigated. Phvul-WOX genes were found to be categorized into three major phylogenetic groups according to the phylogenetic analysis and a total of five segmental duplication events were detected after duplication analysis. Moreover, the Phvul-WOX genes were found to be expressed in different plant tissues at different levels and some stress-related miRNAs have been found to target the Phvul-WOX genes based on miRNA analysis. Additionaly, MDA content, total protein level and catalase enzyme activity analyses were conducted in two P. vulgaris cultivars namely Yakutiye cv. and Zulbiye cv. subjected to 150 mM salt stress. Next, these cultivars were used for screening the expression levels of Phvul-WOX-1, Phvul-WOX-9, Phvul-WOX-11, Phvul-WOX-15 and Phvul-WOX-16 genes in response to salt stress. The insights gained from this study may be of assistance to the researchers who work in this area.