Does continuous flow apheresis influence viability in allogeneic hematopoietic stem cell harvest?
TRANSFUSION AND APHERESIS SCIENCE, vol.34, no.2, pp.171-178, 2006 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 34 Issue: 2
- Publication Date: 2006
- Doi Number: 10.1016/j.transci.2005.12.006
- Journal Name: TRANSFUSION AND APHERESIS SCIENCE
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.171-178
- Ankara University Affiliated: Yes
Abstract
One of the important clinical variables determining the success of hematopoietic stem cell transplantation is the number of viable CD34(+) stem cells transfused to the patient. G-CSF mobilized peripheral blood stem cells from 17 healthy donors were collected by continuous flow apheresis. The median (range) proportions of early apoptotic (Annexin V-FITCpos/7-AAD(neg)) and viable (Annexin V-FITCneg/7-AAD(neg)) CD45(dim)CD34(+) stem cells were 1.5 (0.9-3.7)% and 97.7 (82.8-100)% in the peripheral blood before apheresis and 2.6 (0.8-7.9)% and 97.3 (91.9-99)% in the apheresis products, respectively. Despite an increase in the number of apoptotic cells among all cell compartments, this was statistically significant only in CD34(+) cells and granulocytes. The majority of the cells still retained their viability. (c) 2006 Elsevier Ltd. All rights reserved.