Diabetes decreases mRNA levels of calcium-release channels in human atrial appendage

Guner S., Arioglu E., Tay A., Tasdelen A., Aslamaci S., Bidasee K., ...Daha Fazla

Molecular and Cellular Biochemistry, cilt.263, sa.1, ss.143-150, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 263 Sayı: 1
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1023/b:mcbi.0000041856.92497.0c
  • Dergi Adı: Molecular and Cellular Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.143-150
  • Anahtar Kelimeler: calcium-release channels, diabetes, expression, human atrial appendage, inositol, 1,4,5-trisphosphate receptors, reverse transcription-polymerase chain reaction, ryanodine receptors, INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR, SARCOPLASMIC-RETICULUM CA2+-ATPASE, STAGE HEART-FAILURE, RYANODINE RECEPTORS, CARDIAC MYOCYTES, GENE-EXPRESSION, CA2+ RELEASE, RAT-HEART, CARDIOMYOPATHY, DYSFUNCTION
  • Ankara Üniversitesi Adresli: Evet

Özet

Patients with chronic diabetes mellitus usually develop reductions in rate and force of cardiac contractions. Since calcium-release channels (ryanodine receptors (RyRs) and inositol 1,4,5-trisphosphate receptors (IP3Rs)) play integral roles in effecting these processes, we rationalize that alterations in their expression may underlie these defects. To test this hypothesis, right atrial appendages were obtained from diabetic (65.0 ± 4.5 years) and nondiabetic (56.2 ± 2.6 years) patients undergoing coronary arterial by-pass grafting and reverse transcription-polymerase chain reactions were used to compare steady state levels of mRNA encoding the three major isoforms of RyRs and IP3Rs. In this study we did not detect either RyR1 or RyR3 in human atrial appendage. When compared with nondiabetic patients, mRNA encoding RyR2 from diabetic patients decreased by 74.2 ± 6.2% (p < 0.01). Diabetes also significantly decreased steady-state levels of mRNA encoding the IP3Rs in human atrial appendage. IP3R1 decreased by 24.2 ± 4.6%, IP3R2 decreased by 63.0 ± 4.6% and IP3R3 decreased by 55.5 ± 6.5%. Since a reduction in steady-state mRNA is usually indicative of a decrease in protein levels, these data suggest that the decrease in chronotropy and inotropy seen in chronic diabetic patients may be due in part to a decrease in expression of calcium-release channels. © 2004 Kluwer Academic Publishers.