Akademik Veri Yönetim Sistemi
2nd International Malpighi Symposium, Rome, İtalya, 14 - 16 Eylül 1995, ss.101-108
Confocal microscopy has emerged as a powerful tool for analyzing the regulation of cellular interactions within the mammalian ovary. Using living ol fixed materials labeled with fluorescent probes for organelles, cytoskeletal structures, and cell adhesion molecules, changes in cellular organization during development of the ovarian follicle have been determined. In vitro modifications in the polymerization and organization of actin filaments occur within granulosa cells during the transition from primary (preantral) to secondary (antral) follicles as detected by confocal microscopy in thick frozen sections stained with fluorescent phalloidin. Colocalization with antibodies to phosphotyrosine indicates that cytoskeletal reorganization is restricted to granulosa cells exhibiting heightened levels of protein tyrosine phosphorylation. Striking alterations are apparent within the transzonal projections that conjoin granulosa cells to the oocyte cell surface. Confocal data used to analyze the three dimensional organization of transzonal projections has revealed (1) changes in density and organization during follicle development, (2) species specific variations in cytoskeletal composition and, (3) species specific responses to gonadotropins. These results indicate that gamete somatic cell interactions are dynamically modulated during ovarian follicular growth and oocyte maturation.