Intraspecific Variation within Fusarium oxysporum f. sp cumini from Cuminum cyminum in Turkey

ÖZER G., BAYRAKTAR H.

INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY, cilt.17, sa.2, ss.375-380, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 2
  • Basım Tarihi: 2015
  • Dergi Adı: INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.375-380
  • Anahtar Kelimeler: Cumin, Fusarium wilt, Intraspecific variation, Pathogenicity, PCR-RFLP, INTERGENIC SPACER REGION, RFLP ANALYSIS, GENETIC DIVERSITY, RDNA, IDENTIFICATION, POPULATIONS, FIELDS, ASSAY, ONION, WILT
  • Ankara Üniversitesi Adresli: Evet

Özet

Thirty-nine isolates of Fusarium oxysporum f. sp. cumini were isolated from symptomatic tissues of cumin plants from Ankara and Konya provinces of Turkey during 2011 and 2012 years. Species-specific primers produced a 340-bp DNA fragment from F. oxysporum isolates. All isolates tested for pathogenicity on local cultivar under controlled conditions were highly pathogenic to cumin seedlings, showing variation in their aggressiveness. Also, the restriction digestions of PCR-amplified nuclear ribosomal DNA intergenic spacer (IGS) region were used for determining genetic variability within F. oxysporum f. sp. cumini. Five IGS haplotypes were defined within F. oxysporum f. sp. cumini based on restriction analysis of IGS region with the four restriction enzymes: BsuRI, Tru1I, EcoRI, and MspI. UPGMA analysis revealed four major groups at an arbitrary level of 85% similarity. These findings indicate that isolates of F. oxysporum f. sp. cumini are genetically distinct from each other and the presence of different subgroups of the formae speciales cumini in the important cumin production areas of Turkey. (C) 2015 Friends Science Publishers