Efficient in vitro bulblet regeneration from immature embryos of endangered <i>Sternbergia fischeriana</i>

Mirici S., Parmaksiz I., Ozcan S., SANCAK C., Uranbey S., Sarihan E., ...Daha Fazla

PLANT CELL TISSUE AND ORGAN CULTURE, sa.3, ss.239-246, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1007/s11240-004-3016-y
  • Dergi Adı: PLANT CELL TISSUE AND ORGAN CULTURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.239-246
  • Anahtar Kelimeler: bulblet, embryogenesis, micropropagation, organogenesis, Sternbergia fischeriana, LILY LILIUM-LONGIFLORUM, SOMATIC EMBRYOGENESIS, SHOOT REGENERATION, CALLUS INDUCTION, HIGH-FREQUENCY, PLANT-REGENERATION, EXPLANTS, PROPAGATION, CULTURE, MICROPROPAGATION
  • Ankara Üniversitesi Adresli: Evet

Özet

Sternbergia fischeriana is an endangered geophyte and therefore in vitro micropropagation of this plant will have great importance for germplasm conservation and commercial production. Bulb scale and immature embryo explants of S. fischeriana were cultured on different nutrient media supplemented with various concentrations of plant growth regulators. Immature embryos produced higher number of bulblets than bulb scales. Large numbers of bulblets were regenerated (over 80 bulblets/explants) from immature embryos on Murashige and Skoog (MS) medium supplemented with 4 mg l(-1) 6-benzylaminopurine (BA) and 0.25 mg l(-1) alpha-naphthaleneacetic (NAA) or 2 mg l(-1)2,4-dichlorophenoxyacetic acid (2,4-D) after 14 months of culture initiation. Regenerated bulblets were kept at 5 degreesC for 5 weeks and then transplanted to a potting mixture.