Graphene/Poly(vinylferrocene) Composite Based Amperometric Biosensor for L-lysine Determination

KAÇAR SELVİ C., Erden P. E., Kilic E.

ELECTROANALYSIS, vol.29, no.9, pp.2114-2124, 2017 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 9
  • Publication Date: 2017
  • Doi Number: 10.1002/elan.201700207
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2114-2124
  • Keywords: L-lysine, Amperometry, Biosensor, Graphene, Poly(vinylferrocene), ENZYME ELECTRODE, CARBON NANOTUBES, ALPHA-OXIDASE, PLATINUM NANOPARTICLES, HYDROGEN-PEROXIDE, IMMOBILIZATION, GRAPHENE, SENSORS, QUANTIFICATION, CHROMATOGRAPHY
  • Ankara University Affiliated: Yes

Abstract

A novel and sensitive amperometric biosensor for L-lysine determination based on a glassy carbon electrode (GCE) modified with graphene (GR) and redox polymer poly(vinylferrocene) (PVF) was constructed. L-lysine--oxidase was immobilized onto the modified GCE by a glutaraldehyde/bovine serum albumin cross-linking procedure. SEM, CV and EIS were used for the characterization of the surface morphology and stepwise fabrication processes of PVF/GR composite. Optimal composition of the biosensor and experimental conditions that affect the performance of the biosensor are discussed. The effect of buffer pH on biosensor response was studied in detail over a wide pH range. L-lysine biosensor displayed a linear range of 9.9x10(-7) - 3.1x10(-4) M with a low detection limit of 2.3x10(-7) M and K-M(app) value of 0.4mM. The L-lysine biosensor was tested using pharmaceutical sample and cheese with satisfactory results.