Akademik Veri Yönetim Sistemi
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.60, sa.2, ss.123-128, 2013 (SCI-Expanded)
The objectives of this study were to determine the prevalence of Salmonella in bile of cattle by comparing immunomagnetic separation (IMS) based cultivation and conventional cultivation techniques, to verify the isolates by the detection of oriC gene, to determine phoPQ gene by PCR, to identify the isolates by serotyping and to find out the antibiotic resistance profiles using disc diffusion method. A total of 188 cattle bile samples were collected from two slaughterhouses near Ankara between May-September 2007. In three (1.6 %) of the samples Salmonella spp. were detected by IMS based cultivation technique and two (1.1 %) of the samples with conventional cultivation technique. It was shown that IMS based cultivation technique is more sensitive for the detection of Salmonella in bile of cattle. From the each contaminated samples three colonies were picked and coded (A, B and C). Isolates were verified by detection of oriC gene and phoPQ gene was detected using PCR. According to serotyping; two of them (A and B) were found to be S. Dublin and one (C) S. Bredeney. Disc diffusion method indicated that, S. Bredeney was resistant to ampicillin, cephalothin, tetracycline, nalidixic acid and sulphamethoxazole. All S. Dublin and S. Bredeney isolates were intermediately resistant to streptomycin, also S. Dublin to sulphamethoxazole and S. Bredeney to amoxicillin/clavulanic acid and cephazolin. In conclusion bile can be a site of Salmonella in cattle and all the isolates carried phoPQ gene that may play a significant role in the survival of Salmonella spp. in bile.