[Survey of Theileria annulata and Theileria buffeli/orientalis complex in cattle in the Kirşehir region using multiplex-PCR]. Kirşehir Yöresi Siǧirlarinda Theileria annulata ve Theileria buffeli/ orientalis Varliǧinin Multiplex-PCR Yöntemiyle Araştirilmasi
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneǧi = Acta parasitologica Turcica / Turkish Society for Parasitology, vol.36, no.1, pp.9-11, 2012 (Scopus, TRDizin)
- Publication Type: Article / Article
- Volume: 36 Issue: 1
- Publication Date: 2012
- Doi Number: 10.5152/tpd.20112.03
- Journal Name: Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneǧi = Acta parasitologica Turcica / Turkish Society for Parasitology
- Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
- Page Numbers: pp.9-11
- Ankara University Affiliated: Yes
Abstract
To investigate the presence of T. annulata and T. buffeli/orientalis complex in the blood of 172 cattle selected from 9 different regions of Kirşehir. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with multiplex- PCR for T. annulata and T. buffeli/orientalis, respectively. By multiplex-PCR examination 4 (2.32%) samples were positive for T. annulata whereas none of the samples were positive for T. buffeli/orientalis complex. In this study, it is concluded that simultaneous diagnosis of Theileria species by using multiplex-PCR is more practical than the investigation of species individually by using classical PCR. We also believe that a more accurate epidemiological data is achieved with the use of moleculer diagnosis methods such as multiplex-PCR, which eliminates the disadvantages of the traditional methods used in the diagnosis of parasitic diseases.