Effect of ageing on in vitro endometrial stromal cell differentiation

Atac F., Celikkanat H., Piskin K., Helvacioglu A., Cakar N., BEKSAÇ M., ...Daha Fazla

MEDICAL SCIENCE RESEARCH, cilt.27, sa.12, ss.817-820, 1999 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 12
  • Basım Tarihi: 1999
  • Dergi Adı: MEDICAL SCIENCE RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.817-820
  • Anahtar Kelimeler: ageing, decidualization, differentiation, endometrium, PROLACTIN PRODUCTION, MENSTRUAL-CYCLE, LYMPHOID-CELLS, RECEPTORS, DECIDUALIZATION, PROGESTERONE, PROTEINS
  • Ankara Üniversitesi Adresli: Evet

Özet

Reproductive ageing in the human female is indicated by a decline in fertility. In addition to the decline in oocyte quality, uterine factors which involve both hormonal and stromal-epithelial interactions have vital importance in reduced implantation with ageing. The decidual cells, which have a major role in implantation, are formed by the differentiation of stromal cells. The aim of this study was to analyse the effect of ageing on the decidualization capacity of stroma cells. For this purpose we cultured isolated stromal cells from young (age: 38-42 years) and old (age: 46-52 years) patients in the presence of progesterone, in which they undergo decidual reaction. Secreted prolactin was measured in the spent medium. The mean prolactin amounts were 0.74 +/- 0.09 ng/mu DNA day and 0.79 +/- 0.03 ng/mu DNA day in young and aged groups respectively. The difference was statistically insignificant. We conclude that other cellular and molecular mechanisms must be considered for the role of the uterine factors. Med Sci Res 27:817-820 (C) 1999 Lippincott Williams & Wilkins.