Effect of ageing on in vitro endometrial stromal cell differentiation

Atac F., Celikkanat H., Piskin K., Helvacioglu A., Cakar N., BEKSAÇ M., ...More

MEDICAL SCIENCE RESEARCH, vol.27, no.12, pp.817-820, 1999 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 12
  • Publication Date: 1999
  • Journal Name: MEDICAL SCIENCE RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.817-820
  • Keywords: ageing, decidualization, differentiation, endometrium, PROLACTIN PRODUCTION, MENSTRUAL-CYCLE, LYMPHOID-CELLS, RECEPTORS, DECIDUALIZATION, PROGESTERONE, PROTEINS
  • Ankara University Affiliated: Yes

Abstract

Reproductive ageing in the human female is indicated by a decline in fertility. In addition to the decline in oocyte quality, uterine factors which involve both hormonal and stromal-epithelial interactions have vital importance in reduced implantation with ageing. The decidual cells, which have a major role in implantation, are formed by the differentiation of stromal cells. The aim of this study was to analyse the effect of ageing on the decidualization capacity of stroma cells. For this purpose we cultured isolated stromal cells from young (age: 38-42 years) and old (age: 46-52 years) patients in the presence of progesterone, in which they undergo decidual reaction. Secreted prolactin was measured in the spent medium. The mean prolactin amounts were 0.74 +/- 0.09 ng/mu DNA day and 0.79 +/- 0.03 ng/mu DNA day in young and aged groups respectively. The difference was statistically insignificant. We conclude that other cellular and molecular mechanisms must be considered for the role of the uterine factors. Med Sci Res 27:817-820 (C) 1999 Lippincott Williams & Wilkins.