Four-way parallel factor analysis of voltammetric four-way dataset for monitoring the etoposide-DNA interaction with its binding constant determination

YAZAN Z., Bayraktepe D., DİNÇ E.

Bioelectrochemistry, cilt.134, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 134
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.bioelechem.2020.107525
  • Dergi Adı: Bioelectrochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Etoposide, Deoxyribonucleic acid, Calf thymus DNA, Drug-DNA interaction, PARAFAC, Drug-DNA binding constant, Pencil graphite electrode, FLUORESCENCE SPECTROSCOPY, PARAFAC DECOMPOSITION, ELECTROCHEMICAL APPROACH, CYCLIC VOLTAMMETRY, ANTICANCER DRUGS, MULTIWAY DATA, MATRICES, CHROMATOGRAPHY, COMPLEXES, WATER
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2020 Elsevier B.V.In this paper, a novel strategy in the application of the parallel factor analysis (PARAFAC) to a four-way voltammetric dataset was improved to evidence the interaction of etoposide (ETO) and calf thymus deoxyribonucleic acid (DNA) to determine the ETO-DNA binding constant. PARAFAC is one of the most commonly used techniques applicable to the decomposition of higher-order data arrays to focus on features of interest and provides a different resolution of the chemical problem of interest. Under optimized conditions, peak current data of a seven-sample set containing DNA in the range of 2.0–90.0 µM in the presence of ETO at a constant concentration (10 µM) at five different pHs were recorded as a function of potential and frequency and then arranged as a four-dimensional array. The characteristic curves of ETO and ETO-DNA complex were monitored from the potential, frequency, pH, and DNA concentration profiles obtained by PARAFAC decomposition of the fourth-order array. The binding constant, which is one of the principal parameters for the estimation of drug-DNA interaction and mechanism, was computed from the DNA concentration profile. The consequence of drug-DNA binding constant (K = 1.26 × 106) indicated that there was a significant interaction between ETO and DNA with the intercalation mechanism.