Diagnostic accuracy of refractometry methods for estimating passive immunity status in neonatal beef calves

Akkose M., Buczinski S., ÖZBEYAZ C., Kurban M., Cengiz M., POLAT Y., ...Daha Fazla

VETERINARY CLINICAL PATHOLOGY, cilt.52, sa.1, ss.53-63, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1111/vcp.13171
  • Dergi Adı: VETERINARY CLINICAL PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.53-63
  • Anahtar Kelimeler: beef calves, immunoglobulin G, passive immunity, refractometer, SERUM IMMUNOGLOBULIN-G, OPTICAL REFRACTOMETERS, COLOSTRUM MANAGEMENT, ASSESSING FAILURE, DAIRY CALVES, HEALTH, PERFORMANCE, CALF, ASSOCIATION, PROTEIN
  • Ankara Üniversitesi Adresli: Evet

Özet

Background Assessing the inadequate transfer of passive immunity (ITPI) in beef calves is crucial because calves with ITPI are at high risk for morbidity and mortality. Objectives The aim of this study was to determine the accuracy of digital Brix (D-BRIX) and digital serum total protein (D-STP) refractometers to estimate different passive immunity status in beef calves and to determine the robustness of thresholds. Methods Blood samples were collected from 202 (1-7 days old) beef calves. Serum total solid percentages, total protein concentrations, and IgG concentrations were measured with the D-BRIX refractometer, D-STP refractometer, and gold standard radial immunodiffusion (RID) assay, respectively. Data were analyzed using diagnostic test accuracy, areas under the receiver operating characteristics curve, Cohen's kappa coefficient, and misclassification costs analysis to estimate IgG concentrations <10, <16, and <24 mg/mL. Results For the prediction of serum IgG concentrations <10, <16 and <24 mg/mL, the optimal cut-off values were determined to be <8.5% (Se: 100.0% (95% CI: 87.9-100.0); Sp: 94.2% [95% CI: 89.6-97.2]), <8.5% (Se: 92.1% [95% CI: 78.6-98.2]; Sp: 97.6% [95% CI: 93.9-99.3]), and <10.1% (Se: 88.8% [95% CI: 79.7-94.7]; Sp: 67.2% [95% CI: 58.1-75.4]), respectively, for the D-BRIX refractometer; and <5.2 g/dL (Se: 100.0% [95% CI: 87.9-100.0]; Sp: 93.6% [95% CI: 88.9-96.8]), <5.2 g/dL (Se: 92.1% [95% CI: 78.6-98.2]; Sp: 97.0% [95% CI: 93.0-99.0]), and <6.4 g/dL (Se: 87.5% [95% CI: 78.2-93.8]; Sp: 69.7% [95% CI: 60.7-77.7]), respectively, for the D-STP refractometer. Conclusions The digital Brix and digital serum total protein refractometers can be used as monitoring tools for assessing passive immunity transfer in neonatal beef calves.