Biomimetic 3D-Bone Tissue Model

Parmaksız M., Elçin A. E., Elçin Y. M.

NEXT GENERATION CULTURE PLATFORMS FOR RELIABLE IN VITRO MODELS, cilt.2273, ss.239-250, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 2273
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/978-1-0716-1246-0_17
  • Dergi Adı: NEXT GENERATION CULTURE PLATFORMS FOR RELIABLE IN VITRO MODELS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.239-250
  • Anahtar Kelimeler: 3D bone tissue model, Biomimetic bone scaffold, Decellularization, Extracellular matrix, Composite matrices, Mechanical cue, Mesenchymal stem cell, Regenerative medicine, SMALL-INTESTINAL SUBMUCOSA, OSTEOGENIC DIFFERENTIATION, FLOW PERFUSION, CELLS
  • Ankara Üniversitesi Adresli: Evet

Özet

Various approaches have been evaluated for developing three-dimensional (3D) scaffolds for modeling or engineering of the bone tissue. However, most of such attempts have come up short in mimicking the natural bone tissue extracellular matrix (ECM) microenvironment, especially its natural bioactive content. Here we describe the methodology for the preparation of a natural ECM-based multichannel construct as a biomimetic 3D bone tissue model. We elucidate the construction of the composite scaffold incorporating decellularized small intestinal submucosa ECM, synthetic hydroxyapatite and poly(e-caprolactone), and the mechanical stimulation of the cell-seeded construct under bioreactor culture.