Antibacterial activity on electrospun poly(lactide-co-glycolide) based membranes via Magainin II grafting

Yuksel E., KARAKEÇİLİ A.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, cilt.45, ss.510-518, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.msec.2014.10.004
  • Dergi Adı: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.510-518
  • Anahtar Kelimeler: Magainin II, Antibacterial activity, PLGA, Gelatin, Electrospinning, IN-VITRO ACTIVITY, COVALENT IMMOBILIZATION, ANTIMICROBIAL PEPTIDE, SURFACE, NANOPARTICLES, PREVENTION
  • Ankara Üniversitesi Adresli: Evet

Özet

An antimicrobial peptide (AMP), Magainin II (Mag II) was covalently immobilized on poly(lactide-co-glycolide) (PLGA) and PLGA/gelatin electrospun fibrous membranes. The surface immobilization was characterized by X-ray Photoelectron Spectroscopy (XPS). Scanning Electron Microscopy (SEM) and Atomic Force Microscopy studies showed that the surface morphology of the fibers at micron scale was not affected by the immobilization process. The antibacterial activity of the bound Mag II was tested against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. Bacterial adhesion tests, SEM and confocal analyses revealed that the attachment and survival of bacteria were inhibited on Mag functionalized membranes. AMP immobilization strategy was introduced as a new perspective for the modulation of antibacterial properties on PLGA based materials prepared by electrospinning. (C) 2014 Elsevier B.V. All rights reserved.