Evaluation of the chemopreventive effects of Hypericum perforatum L on DMBA-applied rat oral mucosa

Sengun D. N., Karaca İ. R., Saraç N., Uğur A., Fırat A., Kaymaz F. F., ...Daha Fazla

Archives of Oral Biology, cilt.127, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 127
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.archoralbio.2021.105139
  • Dergi Adı: Archives of Oral Biology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: 7,12-Dimethylbenzanthracene, Oral cancer, Carcinoma in situ, Hypericum perforatum L, Chemoprevention, ST-JOHNS-WORT, FERULIC ACID, CARCINOGENESIS, CANCER, ACTIVATION, EXPRESSION, HYPERFORIN, INDUCTION, DYSPLASIA, APOPTOSIS
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2021 Elsevier LtdObjective: Hypericum perforatum L also known as St. John's wort is known to have many beneficial properties for the organism including its antioxidant and anticancer activities. It is also known to have shown antiproliferative and cytotoxic effects against various cancer cell lines. The purpose of this study was to investigate the effects of Hypericum perforatum L on 7,12-dimethylbenz(a)anthracene-induced rat oral squamous cell carcinoma model. Design: The in vitro antioxidant properties of Hypericum perforatum L was determined and an extract was prepared. Thirty Wistar male rats were divided randomly into 4 groups (Control group, DMBA group, HP + DMBA group, HP group). The antioxidant defense mechanisms in tissue and blood samples were evaluated biochemically and immunohistochemically, the carcinomatous changes in connective tissue were investigated immunohistochemically and epithelial changes in the tissue samples were evaluated histopathologically. Results: The extract revealed inhibitory effects on some antioxidant enzymes (catalase, glutathione peroxidase). Immunohistochemical evaluations revealed no invasive changes in the connective tissue. Hypericum perforatum L demonstrated chemopreventive effects although it did not prevent carcinomatous changes altogether. Conclusions: Hypericum perforatum L is a promising chemopreventive agent and further studies are needed in order to evaluate the full potential of this plant.