EFFECT OF 2,4-DICHLOROPHENOXYACETIC ACID ON THE CHROMOSOME STRUCTURE OF OAT (AVENA SATIVA L.) REGENERANTS

BENLİOĞLU B.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.30, sa.3, ss.2386-2391, 2021 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 3
  • Basım Tarihi: 2021
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2386-2391
  • Anahtar Kelimeler: Avena saliva, aneuploidy, callus induction, choromosome, oat, regenerant, BARLEY HORDEUM-VULGARE, PLANT-REGENERATION, CALLUS INDUCTION, CULTURE, WHEAT
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, the effects of 2,4-Dichlorophenoxyacetic acid (2,4-D), one of the most used growth regulators in plant tissue culture, on oat embryo culture was investigated. Mature embryos of 2 oat cultivars (Faikbey and Seydisehir) were used as explant sources. The effect of, five different doses of 2,4-D (0, 2, 4, 6 and 8 mg 1-1) on callus induction, callus weight, regeneration capacity and culture efficiency parameters were examined in vitro conditions, In the parameters examined for both cultivars, the application of 2 mg 1-1 2,4-D gave the highest results. As a result of cytological studies with root samples obtained from regenerants, it was determined that the dose of 2 mg L1 2,4-D did not cause any change in the number and structure of chromosomes. In oat mature embryo culture studies, it has been determined that a dose of 2 mg 1-1 2.4-D provides the highest tissue culture parameters and can be used safely as it does not cause any somaclonal variation.