Cytotoxicity evaluation of eluates from universal adhesives by real-time cell analysis

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DEMİREL G., Kaya Demirsoy F. F., Irmak O.

DENTAL MATERIALS JOURNAL, cilt.39, sa.5, ss.815-824, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 5
  • Basım Tarihi: 2020
  • Doi Numarası: 10.4012/dmj.2019-221
  • Dergi Adı: DENTAL MATERIALS JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Agricultural & Environmental Science Database, Biotechnology Research Abstracts, Communication Abstracts, Compendex, EMBASE, MEDLINE, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.815-824
  • Anahtar Kelimeler: Cytotoxicity, Universal adhesives, Dental adhesives, Mouse fibroblast cells, Real time cell analysis, DENTIN-BONDING AGENTS, HUMAN GINGIVAL FIBROBLASTS, RESIN COMPONENTS, IN-VITRO, CARBOXYLESTERASE EXPRESSION, URETHANE DIMETHACRYLATE, PULP CELLS, BIOCOMPATIBILITY, COMPOSITE, STRENGTH
  • Ankara Üniversitesi Adresli: Evet

Özet

The aim of this study was to evaluate the cytotoxicity of universal adhesives on L929 mouse fibroblast cell line by using a real-time cell analysis. In order to obtain extract, six different cured dental adhesives were immersed in Dulbecco's Modified Eagle's Medium (DMEM) at 37 degrees C for 24 h. A real-time cell analysis system was used to assess cytotoxicity of the dental adhesives. After seeding 25,000 cells/300 mu L/well cell suspensions into the wells of an e-plate, fibroblasts were exposed to extracts of tested adhesives at varying dilutions (1:1, 1:2, and 1:10) and observed at every 30 min intervals for 72 h. Three-way ANOVA one factor repeated measures were used to analyze the results (alpha=0.05). All tested adhesives induced cell viability loss, cell morphology alteration, and cell death depending on extract concentration and time. Cell viability of L929 cells to between 44 and 10% for 1:1 diluted extracts, at 72 h, when compared to the negative control.