Pathologic Changes and Use of Immunohistochemical Methods in Naturally Occuring Avian Encephalomyelitis of the Chicks

TOPLU N., Alcigir G., TUNCA R.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.15, sa.5, ss.717-724, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 5
  • Basım Tarihi: 2009
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.717-724
  • Anahtar Kelimeler: Avian encephalomyelitis, Avidin-biotin peroxidase complex method, Chick, Immunofluorescence method, EPIDEMIC TREMOR, NERVOUS-SYSTEM, VIRUS, PATHOGENESIS
  • Ankara Üniversitesi Adresli: Evet

Özet

The present study describes the pathological and immunohistochemical findings of avian encephalomyelitis (AE) in naturally infected 31 chicks obtained from three broiler rearing farms. Clinically, ataxia, paresis, paralysis, tremor and torticollis were observed in the chicks. Nonpurulent encephalomyelitis accompanied by neuronal degeneration and necrosis was the most pronounced histopathological findings of the central nervous system (CNS). Lymphoid aggregations and/or mononuclear cell infiltrations were observed in the visceral organs. The avidin-biotin peroxidase complex (ABC) and immunofluorescence (IF) methods were compared for AE viral antigen detection in formalin-fixed, paraffin embedded tissues. With the both tests, condensed viral immunolabelling was detected particularly in the CNS, intestines, proventriculus, kidneys, lungs and heart and, to a lesser extend, in the gizzard, liver, pancreas, spleen, eyes and fabricius of Bursa. Compared of both methods according to labelling intensity and distribution of the viral antigen, ABC method was found to be more sensitive than IF method.