Bu çalışmada beyaz turp (Raphanus sativus var. Longipinnatus) ekstresinin Ehrlich Assit Tümör (EAT) hücre hattı üzerinde zaman ve doza bağlı etkilerinin incelenmesi amaçlanmıştır. Çalışmada EAT hücre hattı kullanıldı. EAT hücreleri, değişen sürelerde (24 ve 48 saat) ve dozlarda (100-200 ve 300 μg/ml beyaz turp ekstresi) 37°C ve %5 CO2'de beyaz turp ekstresine (Raphanus sativus var. Longipinnatus) maruz bırakıldı. İnkübasyon süresinin sonunda EAT hücrelerinin argyrophilic nükleolar organize bölge (AgNOR) protein durumu incelendi. 48 saatlik inkübasyonda ortalama AgNOR sayısı ve TAA/NA açısından kontrol ve 300 μg/ml Raphanus sativus ekstresi grubu arasında anlamlı fark bulundu. Ayrıca 48 saatlik inkübasyonda AgNOR sayısı ve TAA/NA için 100 ve 300 μg/ml Raphanus sativus ekstre gruplarında istatistiksel olarak fark görüldü (p
In this research, it was aimed to research the time and different dose effects of white radish extract (Raphanus sativus var. Longipinnatus) on Ehrlich Ascites Tumor (EAT) cell lines. EAT cell line was used in the study. EAT cells were treated white radish extract (Raphanus sativus var. Longipinnatus) at 37°C and 5% CO2 for differing periods (24 and 48 hours) and doses (100-200 and 300 μg/ml white radish extract). At the end of the incubation duration, Argyrophilic nucleolar organizing region (AgNOR) protein condition of EAT cells were investigated.It was determined among the control and 300 μg/ml Raphanus sativus extract group the significant differences for mean AgNOR number and TAA/NA in 48 hours period. It was detected also between the 100 and 300 μg/ml Raphanus sativus extract groups for AgNOR number and TAA/NA in 48 hours incubation. This study demonstrated that Raphanus sativus had a important role against cancer cells. Also, both AgNOR values mit be used as biomarkers for identification of the most true therapeutic dose option for cancer and it has been shown that suitable ingestion of Raphanus sativus can be effective in avoid cancer development and slowing its spreading.
