Akademik Veri Yönetim Sistemi
4th INTERNATIONAL CONGRESS OF VETERINARY BIOCHEMISTRY AND CLINICAL BIOCHEMISTRY, Sivas, Türkiye, 4 - 07 Eylül 2025, ss.38-44, (Tam Metin Bildiri)
Introduction and Purpose: Cardamom is a spice belonging to the Zingiberaceae family and contains bioactive compounds such as limonene, cymene, pinene, linalool, borneol, cardamonin, indole-3-carbinol, diindolylmethane, α-terpenyl acetate and 1,8-cineole. It is known to provide numerous health benefits such as antioxidant, antimicrobial, anti-inflammatory, antidiabetic, and cancer risk reducing potential due to the bioactive compounds it contains. This investigation focused on the evaluation of total phenolic and flavonoid content, antioxidant capacity, and the anticancer activity of cardamom oil in the MEC-1 cell line. Materials and Methods: The DPPH radical scavenging activity, total polyphenol and flavonoid content of cardamom oil methanolic extract were analyzed spectrophotometrically. The effects of cardamom oil on MEC-1 cell line were evaluated using MTT method. Results: In the methanol extract of cardamom oil, total flavonoid content was found as 20.92 mg/g RE (Rutin Equivalent), total polyphenol substance content as 4.05 mg/g GAE (Gallic Acid Equivalent), and DPPH radical scavenging activity as 0.01 ng/g GAE. For MTT analysis, cardamom oil was applied to the MEC-1 cell line at different concentrations (100-0 mg/ml). The most pronounced cytotoxic effect was observed at a concentration of 100 mg/ml, resulting in a reduction in cell viability to 43.99%. Following application of cardamom oil at a concentration of 50 mg/ml, 53.325% cell viability was observed in the MEC-1 cell line. A dose-dependent decrease in cell proliferation was observed. Discussion and Conclusion: The dose-dependent reduction observed in cancer cell proliferation following cardamom oil treatment is a promising indicator of its anticancer activity and is consistent with previous studies reporting the cytotoxic effects of cardamom oil on various cancer cell lines. Further research is needed to fully explore the therapeutic potential of cardamom oil, particularly given its anticancer effects. In conclusion, this study is significant in demonstrating the health benefits of cardamom oil, especially its therapeutic potential in cancer treatment.