Akademik Veri Yönetim Sistemi
Journal of the Electrochemical Society, cilt.172, sa.6, 2025 (SCI-Expanded)
Acute myocardial infarction (AMI) is a critical cardiovascular disease characterized by myocardial necrosis. Cardiac troponin T (cTnT), released into the bloodstream upon myocardial injury, leading to elevated protein concentrations, is widely recognized as the gold-standard biomarker for AMI diagnosis. Here, we developed a novel aptasensor for cTnT detection, exhibiting high efficiency, sensitivity, and selectivity at femtomolar concentrations. The aptasensor was fabricated by electroplating graphene oxide and gold nanoparticles onto a screen-printed electrode, significantly enhancing its electrochemical activity. Under optimal conditions, the aptasensor demonstrated a broad linear detection range of 0.245 fM-1 μM, with a detection limit of 0.082 fM. It also exhibited excellent selectivity in the presence of several interfering compounds and remarkable reproducibility and stability. Furthermore, the aptasensor was evaluated using human serum and fetal bovine serum samples across various concentrations, and the results were validated via ELISA assay. The obtained critical t-value (tcritical = 2.13) indicated no statistically significant difference between the two methods. In conclusion, the developed aptamer-based sandwich assay enables highly sensitive and selective detection of cTnT at femtomolar levels in real samples. This sensor platform would be a candidate for the rapid and straightforward analysis of cTnT in clinical diagnostics for many biomarkers.