Determination of acid α-naphthyl acetate esterase enzyme activity in peripheral blood leukocytes of gazelles (Gazella subgutturosa)

Altunay H., Harem I., Harem M. K., Asti R. N., Kurtdede N.

Biotechnic and Histochemistry, vol.83, no.6, pp.279-283, 2008 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 83 Issue: 6
  • Publication Date: 2008
  • Doi Number: 10.1080/10520290802602075
  • Journal Name: Biotechnic and Histochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.279-283
  • Keywords: alpha-naphthyl acetate asterase, ANAE, gazelle, peripheral blood, LYMPHOCYTES-T, IDENTIFICATION, CATTLE, CELLS, SUBPOPULATIONS, THYMOCYTES, MONOCYTES, CHICKEN, MARKER
  • Ankara University Affiliated: Yes

Abstract

We examined gazelle peripheral blood leucocytes using the α-Naphthyl acetate esterase (ANAE) staining technique (pH 5.8). Our purpose was to determine the percentage of ANAE positive lymphocytes. The proportion of ANAE positive T-lymphocytes was 72%. T-lymphocytes showed an ANAE positive reaction, but eosinophilic granulocytes and monocytes also showed a positive reaction. By contrast, no reaction was detected in B-lymphocytes, neutrophil granulocytes or platelets. The reaction observed in T-lymphocytes was a red-brown coloration, usually 1-2 granules, but enough granules to fill the cytoplasm were detected rarely. As a result of ANAE enzyme staining, we concluded that the staining technique can be used as a cytochemical marker for gazelle T-lymphocytes.