Determination of acid α-naphthyl acetate esterase enzyme activity in peripheral blood leukocytes of gazelles (Gazella subgutturosa)
Biotechnic and Histochemistry, cilt.83, sa.6, ss.279-283, 2008 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 83 Sayı: 6
- Basım Tarihi: 2008
- Doi Numarası: 10.1080/10520290802602075
- Dergi Adı: Biotechnic and Histochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.279-283
- Anahtar Kelimeler: alpha-naphthyl acetate asterase, ANAE, gazelle, peripheral blood, LYMPHOCYTES-T, IDENTIFICATION, CATTLE, CELLS, SUBPOPULATIONS, THYMOCYTES, MONOCYTES, CHICKEN, MARKER
- Ankara Üniversitesi Adresli: Evet
Özet
We examined gazelle peripheral blood leucocytes using the α-Naphthyl acetate esterase (ANAE) staining technique (pH 5.8). Our purpose was to determine the percentage of ANAE positive lymphocytes. The proportion of ANAE positive T-lymphocytes was 72%. T-lymphocytes showed an ANAE positive reaction, but eosinophilic granulocytes and monocytes also showed a positive reaction. By contrast, no reaction was detected in B-lymphocytes, neutrophil granulocytes or platelets. The reaction observed in T-lymphocytes was a red-brown coloration, usually 1-2 granules, but enough granules to fill the cytoplasm were detected rarely. As a result of ANAE enzyme staining, we concluded that the staining technique can be used as a cytochemical marker for gazelle T-lymphocytes.