Geobacillus thermodenitrificans subsp calidus, subsp nov., a thermophilic and alpha-glucosidase producing bacterium isolated from Kizilcahamam, Turkey

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Cihan A., Ozcan B., Tekin N., Cokmus C.

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, cilt.57, sa.2, ss.83-92, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57 Sayı: 2
  • Basım Tarihi: 2011
  • Doi Numarası: 10.2323/jgam.57.83
  • Dergi Adı: JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.83-92
  • Anahtar Kelimeler: DNA-DNA hybridization, genomic fingerprinting, Geobacillus, lipid, 16S rRNA gene, taxonomy, thermostable alpha-glucosidase, BACILLUS-THERMODENITRIFICANS, DEOXYRIBONUCLEIC-ACID, DNA HYBRIDIZATION, LIPID-COMPOSITION, RAPID METHOD, THERMOGLUCOSIDASIUS, THERMOCATENULATUS, THERMOLEOVORANS, PCR
  • Ankara Üniversitesi Adresli: Evet

Özet

An alpha-glucosidase producing, thermophilic, facultatively anaerobic, and endospore-forming, motile, rod-shaped bacterial strain F84b(T) was isolated from a high temperature well-pipeline sediment sample in Kizilcahamam, Turkey. The growth occurred at temperatures, pH and salinities ranging from 45 to 69 degrees C (optimum 60 degrees C), 7.0 to 8.5 (optimum 8.0) and 0 to 5% (w/v) (optimum 3.5%), respectively. Strain F84b(T) was able to grow on a wide range of carbon sources. Starch and tyrosine utilization, amylase, catalase and oxidase activities, nitrate reduction, and gas production from nitrate were all positive. The G+C content of the genomic DNA was 49.6 mol%. The menaquinone content was MK-7. The dominant cellular fatty acids were iso-C17:0, iso-C15:0, and C16:0. In phylogenetic analysis of 16S rRNA gene sequence, strain F84b(T) showed high sequence similarity to Geobacillus thermodenitrificans (99.8%) and to Geobacillus subterraneus (99.3%) with DNA hybridization values of 74.3% and 29.1%, respectively. In addition, the Rep-PCR and the intergenic 16S-23S rRNA gene fingerprinting profiles differentiated strain F84b(T) from the Geobacillus species studied. The results obtained from the physiological and biochemical characters, the menaquinone contents, the borderline DNA-DNA hybridization homology, and the genomic fingerprinting patterns had allowed phenotypic, chemotaxonomic and genotypic differentiation of strain F84b(T) from G. thermodenitrificans. Therefore, strain F84b(T) is assigned to be a new subspecies of G. thermodenitrificans, for which the name Geobacillus thermodenitrificans subsp. calidus, subsp. nov. is proposed (The type strain F84b(T) = DSM 22629(T) = NCIMB 14582(T)).