Akademik Veri Yönetim Sistemi
VI. International Agricultural, Biological & Life Science Conference, Antalya, Türkiye, 30 Ekim - 01 Kasım 2024, cilt.1, sa.1, ss.169, (Özet Bildiri)
The Late Embryogenesis Abundant (LEA) gene family is vital for many developmental and growth processes in plants. Its members play important roles in responses to abiotic stressors including freezing and desiccation. Due to their notable hydrophilicity and thermal stability characteristics, LEA proteins are essential for preserving cell membrane integrity, forming molecular barriers, aiding in ionic binding, and mitigating oxidative damage during extended periods of exposure to abiotic stress conditions. Although LEA proteins have been extensively studied in numerous plant species so far, this study represents the first comprehensive exploration and characterization of LEA proteins in Phaseolus vulgaris genome. In this context, the biochemical/physicochemical properties of the LEA gene family at both the gene and protein level have been deeply characterized and defined using various bioinformatics tools. Through comprehensive bioinformatics analyses, we identified 80 LEA genes and phylogenetically categorized their proteins into eight major groups specific to the common bean. Our phylogenetic analysis highlighted LEA_2 as the subfamily with the highest member count. Investigating gene duplications, we uncovered 28 events, including 24 segmental and 4 tandem duplications, significantly influencing the evolutionary trajectory of this gene family. In silico micro-RNA (miRNA) target analyses revealed that 21 PvLEA genes were targeted by various miRNAs, with miRN2588 and mir164 being the most prevalent. PvLEA-63 emerged as the most highly expressed gene across tissues, followed by PvLEA-27, PvLEA-35, PvLEA-41, PvLEA-49, and PvLEA-52, demonstrating their ubiquitous expression patterns. Moreover, using publicly available RNAseq data, a comparative expression study of PvLEA genes was carried out, and expression alterations in PvLEA-02, -08, -20, -21, -40, -42, -50, and -51 genes were detected under both salt and drought stress conditions. Furthermore, qRT-PCR analysis of PvLEA genes was conducted in P. vulgaris subjected to salt stress in order to confirm the data derived from RNAseq analysis. To sum up, it was seen that some of the PvLEA genes might play important roles for abiotic stress defence in P. vulgaris. The results obtained from this study might constitute a substantial resource for future researchers interested in unraveling the functional intricacies of PvLEA genes