Akademik Veri Yönetim Sistemi
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.55, sa.3, ss.167-171, 2008 (SCI-Expanded)
The aim Of this Study was to investigate the clinical samples obtained from cats and dogs for dermatophyte identification by direct microscopy, dermatophyte isolation by cultural methods, respectively and to confirm these by molecular methods. A total of 268 material was examined for the isolation of dermatophyte. Fungal hyphas and/or spores were observed in 39 (14.5 %) out of 268 materials following direct microscopy. Dermatophyte isolations were achieved from 40 (14.9 %) materials after the Culture process. Considering the origins of materials examined, dermatophyte isolation ratios were 42 % ill cat materials and 7.5 % in dogs'. Higher isolation rates were achieved on dermatophyte test medium (DTM) than sabouraud dextrose agar (SDA) after studies. The distribution of isolated strains according to the species of animals was determined to be 95.9 % Microsporum canis and 4.1 % Microsporum nanum in cats; 50 % Microsporum canis, 18.7 % Trichophyton mentagrophytes, 12.5 % Trichophyton terrestre, 12.5 Microsporum gypseum and 6.3 % Microsporum nanum in dogs. In this study, no significant effect of sex on dermatophytosis prevalence (p>0.05) was detected in cats, while the prevalences were found to be significant (p <= 0.01) in animals (cats and/or) those were smaller than I year old compared to the animals of other age groups. It was determinated that all of the isolated strains were determined to be dermatophyte species following PCR studies. As a conclusion, Microsporum canis was the dominantly isolated agent from dermatophytosis suspected cats and dogs and DTM was determined to be more effective than SDA concerning dermatophyte isolations. The feasibility of molecular techniques for confirmation of laboratory diagnosis of isolated strains had been shown. It was also concluded that, owners and veterinarians should be aware and careful when they are in contact with suspected animals, concerning the zoonotical importance of Microsporum canis.