Impact of harvest product volume in erythrocyte depletion of allogeneic or autologous bone marrow using COBE spectra

Soydan E., Ayyildiz E., Dalva K., Topcuoglu P., Tol M., Arslan O., ...More

TRANSFUSION AND APHERESIS SCIENCE, vol.36, no.3, pp.269-273, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1016/j.transci.2007.03.006
  • Journal Name: TRANSFUSION AND APHERESIS SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.269-273
  • Keywords: erythrocyte depletion, cell separator, bone marrow, harvest volume, STEM-CELL TRANSPLANTATION, MONONUCLEAR-CELLS, SEPARATOR, BLOOD, IMPLANTATION, GRAFTS
  • Ankara University Affiliated: Yes

Abstract

Depletion of bone marrow (BM) from erythrocytes is used to prevent early hemolysis in major ABO incompatible allogeneic hematopoictic cell transplantation (Allo-HCT). This method was also strongly recommended before storing of autologous and even allo-BM for volume reduction in order to prevent early hemolysis and DMSO toxicity after infusion. In our center, erythrocyte depletion of BM harvests has been performed on a continuous flow cell separator, which used a closed system with a high mononuclear cell (MNC) yield and low rate of erythrocyte contamination. According to the protocol of a cellular therapy approach in a cardiovascular collaborative study we have to adopt the process to lower volumes. We aimed to compare our results with standard volume (SV) (historical control) to low volume ED procedures.