Impact of harvest product volume in erythrocyte depletion of allogeneic or autologous bone marrow using COBE spectra

Soydan E., Ayyildiz E., Dalva K., Topcuoglu P., Tol M., Arslan O., ...Daha Fazla

TRANSFUSION AND APHERESIS SCIENCE, cilt.36, sa.3, ss.269-273, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 3
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.transci.2007.03.006
  • Dergi Adı: TRANSFUSION AND APHERESIS SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.269-273
  • Anahtar Kelimeler: erythrocyte depletion, cell separator, bone marrow, harvest volume, STEM-CELL TRANSPLANTATION, MONONUCLEAR-CELLS, SEPARATOR, BLOOD, IMPLANTATION, GRAFTS
  • Ankara Üniversitesi Adresli: Evet

Özet

Depletion of bone marrow (BM) from erythrocytes is used to prevent early hemolysis in major ABO incompatible allogeneic hematopoictic cell transplantation (Allo-HCT). This method was also strongly recommended before storing of autologous and even allo-BM for volume reduction in order to prevent early hemolysis and DMSO toxicity after infusion. In our center, erythrocyte depletion of BM harvests has been performed on a continuous flow cell separator, which used a closed system with a high mononuclear cell (MNC) yield and low rate of erythrocyte contamination. According to the protocol of a cellular therapy approach in a cardiovascular collaborative study we have to adopt the process to lower volumes. We aimed to compare our results with standard volume (SV) (historical control) to low volume ED procedures.