Nanomaterial-based sandwich-type electrochemical aptasensor platform for sensitive voltammetric determination of leptin


Erkmen C., AYDOĞDU TIĞ G., USLU B.

MICROCHIMICA ACTA, cilt.189, sa.10, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 189 Sayı: 10
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s00604-022-05487-z
  • Dergi Adı: MICROCHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Anahtar Kelimeler: Aptasensor, Differential pulse voltammetry, Gold nanoparticles, Leptin, Zinc oxide nanoparticles, HEMIN/G-QUADRUPLEX DNAZYMES, AU NANOPARTICLES, GOLD NANOPARTICLES, GRAPHENE OXIDE, IMMUNOSENSOR, SERUM, AMPLIFICATION, BIOSENSOR, SENSOR
  • Ankara Üniversitesi Adresli: Evet

Özet

A sandwich-type electrochemical aptasensor was designed for sensitive detection of leptin in biological samples, including human serum and human plasma. The developed aptasensor was produced by electrodeposition of gold nanoparticles on a screen-printed electrode modified with zinc oxide nanoparticles. The synergy effect of zinc oxide and gold nanoparticles improved the electrocatalytic activity of the aptasensor. The obtained high surface area allowed more aptamer molecules to be loaded on the electrode surface. Signal amplification significantly increases the detection sensitivity of a developed biosensor. Although the use of nanomaterials is the most preferred detection tool for this purpose, as an alternative, enzyme-catalyzed signal amplification is widely used in the construction of a biosensor due to its specificity and high catalytic efficiency. Therefore, both nanomaterial-supported and an alkaline phosphatase-based aptasensor design were developed, which can produce in situ electroactive product by enzymatic hydrolysis of the inactive substrate to achieve a higher signal-to-background ratio. Under optimal conditions, the developed aptasensor exhibited a wide linear concentration range from 0.01 pg mL(-1) to 100.0 pg mL(-1) with a detection limit of 0.0035 pg mL(-1). While the developed aptasensor provided excellent selectivity in the presence of some interfering compounds, it possessed outstanding reproducibility and stability. In addition, the developed aptasensor has been applied with good recoveries in the range 96.31 to 108.79% in human serum and plasma samples. In conclusion, all the obtained results showed the feasibility of the developed aptasensor for practical applications.