Akademik Veri Yönetim Sistemi
TURKISH JOURNAL OF BOTANY, cilt.49, ss.1-9, 2025 (SCI-Expanded)
The widespread utilisation of deep sequencing has dramatically enhanced and revolutionised our understanding of virus
diversity, particularly in complex environments such as soil and biological samples. This research explored mycovirus sequence analyses
in the ectomycorrhizal fungus Amanita vidua, utilising deep sequencing alongside total double-stranded RNA (dsRNA) extractions.
Our investigation unveiled a unique member of the proposed family, Narliviridae, designated as Amanita vidua narlivirus 1 (AvNV1),
in an A. vidua specimen. AvNV1, classified under Amabiliviricetes, possesses a distinct genome structure and sequence attributes.
Thorough analyses indicated that AvNV1 exhibits sequence similarities with other mycoviruses from the proposed family Narliviridae
and forms a cluster within an as-yet-unclassified genus. The identification of AvNV1 contributes to the genetic variety of mycoviruses in
the Amabiliviricetes category, providing insights into their evolutionary trajectory. In conclusion, this study underscores the significance
of delving into viral diversity in noncultivatable fungi, filling in gaps in our understanding of mycovirus ecology. Detecting a new
narlivirus expands our knowledge of mycovirus diversity and evolution, illustrating the necessity for ongoing research into viral
communities in noncultivatable fungi.