Effects of adrenomeduline and ramp2 on the lung of mice exposed to total body radiation

Ozbilgin M. K., Karaman G. Z., Gencur S., Gumustepe E., Kurtman C.

INTERNATIONAL JOURNAL OF RADIATION RESEARCH, cilt.18, sa.3, ss.571-578, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.18869/acadpub.ijrr.18.3.571
  • Dergi Adı: INTERNATIONAL JOURNAL OF RADIATION RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.571-578
  • Anahtar Kelimeler: AM, RAMP2, radiation, lung, carcinogens, ENDOTHELIAL ADRENOMEDULLIN-RAMP2 SYSTEM, HYPOTENSIVE PEPTIDE, VASCULAR INTEGRITY, CANCER CELLS, KAPPA-B, GROWTH, ANGIOGENESIS, EXPRESSION, VEGF
  • Ankara Üniversitesi Adresli: Evet

Özet

Background: Adrenomedullin (AM) and its receptor, receptor activity-modifying protein (RAMP) 2 have pleiotropic regulatory functions in normal tissue and cancer tissue. AM is produced and secreted both numerous stromal cells and tumor cells. This study aims to investigate a possible role of AM and RAMP2 in the radiation exposure in the normal lung tissue. Materials and Methods: Four groups with 6 male adult Swiss Albino mice per group were investigated. The mice were subjected to a 500 cGy single-dose radiation exposure in the total body radiation device and lung tissues were collected. 1, 2, and 7 days after radiation exposure, with 1 reference group which was not exposed to radiation. Results: The general histology and the immunohistochemistry of the tissue samples prepared with anti-AM, anti-RAMP2, and monoclonal antibodies were investigated, yielding a statistically significant increase for AM on day 3 and for RAMP2 on day 1 after radiation exposure. Conclusion: The observed increase of AM and RAMP2 concentrations in the normal tissue matrix after radiation exposure may play a role in the side effects of radiotherapy.