Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.73, sa.3, ss.253-266, 2016 (Scopus)
Objective: The aim of the study is to determine the genotoxic effects of various environmental pollutants around cement factory in Askale-Erzurum. It was studied four lichen species which include Pseudevernia furfuracea, Lobaria pulmonaria, Cetralia islandica and Usnea longissima. Methods: The main observation or changes in the protein assay and RAPD patterns included appearance of new bands and/or disappearance of normal bands compared with the control samples. Results: Although significant amount of decrease in protein content of the samples exposed to pollutants has been observed 50 m away from cement factory, no changes was detected in the protein content of liken samples 100 m and 200 m away from cement factory. Among the four studied species, P. furfuracea revealed to have the highest level of band appearance and disappearance. Following the exposure to the pollutants of 1, 2 and 3 district situated at a distance of 50, 100, 200m to the cement factory, P. furfuracea with a control bands were observed respectively. Moreover 31, 13 and 15 bands from the control species disappeared in sites 1, 2 and 3 in P. furfuracea samples. Furthermore, the highest polymorphism value was obtained (P% = 86,6%) in U. longissima and L. pulmonaria by the OPC04 primer, and the lowest polymorphism was yielded (P%= 45,4%) in L. pulmonaria by the OPC01 primer. According to this study site 1, which is the nearest site to the cement factory (50m), has the highest appearance and disappearance band. As the samples from site 1 revealed the lowest level of GTS values might led to a high level of genotoxic effect in the four lichen species. Conclusion: This study provides preliminary evidence to the biological effects and genotoxicological consequences caused by various environmental contaminants with the use of four different lichen species collected from around cement factory. The use of indicator organisms as a biomarker in the early detection of genotoxic agents showed reliable sensitivity in terms of estimating the level of damage caused by air pollution.