Nanoliter droplet vitrification for oocyte cryopreservation

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Zhang X., Khimji I., Shao L., Safaee H., Desai K., Keles H. O., ...Daha Fazla

NANOMEDICINE, cilt.7, sa.4, ss.553-564, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.2217/nnm.11.145
  • Dergi Adı: NANOMEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.553-564
  • Anahtar Kelimeler: cryopreservation, fertility, nanoliter droplet vitrification, oocyte, MATURE MOUSE OOCYTES, OPEN PULLED STRAWS, BOVINE OOCYTES, MEIOTIC SPINDLES, CYTOSKELETAL ORGANIZATION, FERTILITY PRESERVATION, CONVENTIONAL STRAWS, OPS VITRIFICATION, LIQUID-NITROGEN, SURVIVAL
  • Ankara Üniversitesi Adresli: Hayır

Özet

Aim: Oocyte cryopreservation remains largely experimental, with live birth rates of only 2-4% per thawed oocyte. In this study, we present a nanoliter droplet technology for oocyte vitrification. Materials & methods: An ejector-based droplet vitrification system was designed to continuously cryopreserve oocytes in nanoliter droplets. Oocyte survival rates, morphologies and parthenogenetic development after each vitrification step were assessed in comparison with fresh oocytes. Results: Oocytes were retrieved after cryoprotectant agent loading/unloading, and nanoliter droplet encapsulation showed comparable survival rates to fresh oocytes after 24 h in culture. Also, oocytes recovered after vitrification/thawing showed similar morphologies to those of fresh oocytes. Additionally, the rate of oocyte parthenogenetic activation after nanoliter droplet encapsulation was comparable with that observed for fresh oocytes. This nanoliter droplet technology enables the vitrification of oocytes at higher cooling and warming rates using lower cryoprotectant agent levels (i.e., 1.4 M ethylene glycol, 1.1 M dimethyl sulfoxide and 1 M sucrose), thus making it a potential technology to improve oocyte cryopreservation outcomes.