Effect of different extenders and individuals in freezing of dog semen

Tosun H., UYSAL O.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.54, sa.1, ss.23-28, 2007 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 1
  • Basım Tarihi: 2007
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.23-28
  • Anahtar Kelimeler: bovine serum albumine, cryopreservation, dog, extender, semen, MEMBRANE INTEGRITY, SEMINAL PLASMA, INSEMINATION, PRESERVATION, INTRAUTERINE, MOTILITY, ALBUMIN
  • Ankara Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate the effect of different semen extender and bovine serum albumine added to extender on freezing of dog semen. In study, 5 German Shepherd dog used for semen collecting were 2-4 years old. During research, a total of 50 ejaculates consist of 10 samples from each dog by massage method twice in a week. Differences among dogs for sperm motility and sperm concentration were significant (p < 0.01) and no differences among dogs for other sperm characteristics were found significant (p > 0.05) in fresh semen statistically. Post-thawing sperm motility (%) was found 58.0 +/- 11.3, 55.8 +/- 10.8, the percentage of abnormal sperm(%) 11.1 +/- 5.2, 18.3 +/- 4.5 and the percentage of dead sperm (%) 29.8 +/- 5.5 and 37.4 +/- 6.0 for mtris A (glycerol, BSA, yumurta sarisi) and mtris B (glycerol, egg yolk) in frozen dog semen. respectively. The same spermatological characteristics (%) were recorded as 34.0 +/- 9.8, 36.0 +/- 8.7; 11.2 +/- 4.2, 11.0 +/- 3.2 and 27.7 +/- 8.6 and 28.4 +/- 9.4 for laiciphos-A (glycerol, BSA, yumurta sarisi) and for laiciphos-B (glycerol, egg yolk) respectively. Differences among extenders for post-thawing sperm motility, the percentage of abnormal sperm and the percentage of dead sperm were found significant statistically (p < 0.001, p < 0.01).