In vitro evaluation of encapsulated primary rat hepatocytes pre- and post-cryopreservation at-80 degrees C and in liquid nitrogen

Durkut S., Elçin A. E., Elçin Y. M.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.43, sa.1, ss.50-61, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3109/21691401.2013.837476
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.50-61
  • Anahtar Kelimeler: alginate, bioartificial liver, CYP450 activity, chitosan, cryopreservation, hepatocyte, encapsulation, synthetic metabolism, xenobiotic metabolism, MAMMALIAN-CELL ENCAPSULATION, ACUTE LIVER-FAILURE, MICROENCAPSULATED HEPATOCYTES, BIOARTIFICIAL LIVER, CHITOSAN-ALGINATE, MICROCAPSULES, TRANSPLANTATION, VIABILITY, CULTURE, PRESERVATION
  • Ankara Üniversitesi Adresli: Evet

Özet

Encapsulation techniques have the potential to protect hepatocytes from cryoinjury. In this study, we comparatively evaluated the viability and metabolic function of primary rat hepatocytes encapsulated in calcium alginate microbeads, in chitosan tripolyphosphate beads, and in three-layered alginate-chitosan-alginate (ACA) microcapsules, before and after cryopreservation at -80 degrees C and in liquid nitrogen (LN2) for 1 and 3 months. Findings demonstrated that LN2 was atop of -80 degrees C in regard to preservation of viability (> 90%) and hepatic functions. LN2-cryopreserved hepatocytes encapsulated in ACA microcapsules retained metabolic function post-thawing, with > 90% of the albumin, total protein and urea syntheses activities, and > 80% of oxidative function.