Comparative study of the ratio spectra derivative spectrophotometry, derivative spectrophotometry and Vierordt's method applied to the analysis of oxfendazole and oxyclozanide in a veterinary formulation
ANALUSIS, cilt.25, sa.3, ss.55-59, 1997 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 25 Sayı: 3
- Basım Tarihi: 1997
- Dergi Adı: ANALUSIS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.55-59
- Anahtar Kelimeler: oxfendazole, oxyclozanide, veterinary formulation, ratio spectra derivative spectrophotometry, derivative spectrophotometry, Vierordt's method, LIQUID-CHROMATOGRAPHIC METHOD, MUSCLE
- Ankara Üniversitesi Adresli: Evet
Özet
Three new spectrophotometric methods are described for the determination of oxfendazole and oxyclozanide in their binary mixture: first derivative spectrophotometry, ratio spectra derivative and Vierordt's method. The procedures do not require any prior separation. In the derivative spectrophotometry, the dA/d lambda. values were measured at 308.8 nm for oxyclozanide and at 326.0 nm for oxfendazole in the first derivative spectra of the mixture. The calibration graphs were linear in the range 8-32 mu g.mL(-1) for oxyclozanide and 4-16 mu g.mL(-1) for oxfendazole. In ratio spectra derivative spectrophotometry, the calibration graphs for 4-32 mu g.mL(-1) oxyclozanide and for 2-16 mu g.mL(-1) oxfendazole were obtained by measuring the signals at 255.0 and 273.1 nm fog oxyclozanide and at 281.8 and 304.6 nm for oxfendazole. In Vierordt's method, A(1)(1) (%1, 1 cm) values of oxfendazole and oxyclozanide were determined at 308.7 and 326.1 nm in the zero-order spectra. The amounts of both compounds were calculated by using the A(1)(1) (%1, 1 cm) values. The methods were successfully applied to a veterinary formulation for determination of both active compounds.