Decellularized Cell Culture ECMs Act as Cell Differentiation Inducers

Parmaksız M., Elçin A. E., Elçin Y. M.

STEM CELL REVIEWS AND REPORTS, cilt.16, sa.3, ss.569-584, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s12015-020-09963-y
  • Dergi Adı: STEM CELL REVIEWS AND REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.569-584
  • Anahtar Kelimeler: Cell culture ECM, Decellularization, Cell differentiation inducer, Tissue engineering, Regenerative biomaterials, In vitro cell culture model, MESENCHYMAL STEM-CELLS, VON-WILLEBRAND-FACTOR, EXTRACELLULAR-MATRIX, OSTEOGENIC DIFFERENTIATION, GROWTH-FACTORS, TISSUE, MARKERS, FIBROBLAST, EXPRESSION, SCAFFOLD
  • Ankara Üniversitesi Adresli: Evet

Özet

Decellularized tissues and organs have aroused considerable interest for developing functional bio-scaffolds as natural templates in tissue engineering applications. More recently, the use of natural extracellular matrix (ECM) extracted from the in vitro cell cultures for cellular applications have come into question. It is well known that the microenvironment largely defines cellular properties. Thus, we have anticipated that the ECMs of the cells with different potency levels should likely possess different effects on cell cultures. To test this, we have comparatively evaluated the differentiative effects of ECMs derived from the cultures of human somatic dermal fibroblasts, human multipotent bone marrow mesenchymal stem cells, and human induced pluripotent stem cells on somatic dermal fibroblasts. Although challenges remain, the data suggest that the use of cell culture-based extracellular matrices perhaps may be considered as an alternative approach for the differentiation of even somatic cells into other cell types.