Impact of lactate in the perfusate on function and metabolic parameters of isolated working rat heart

BEŞİKCİ A.

MOLECULAR AND CELLULAR BIOCHEMISTRY, cilt.296, sa.1-2, ss.121-127, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 296 Sayı: 1-2
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1007/s11010-006-9305-5
  • Dergi Adı: MOLECULAR AND CELLULAR BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.121-127
  • Anahtar Kelimeler: cardiac metabolism, fatty acid oxidation, lactate oxidation, FATTY-ACID OXIDATION, ACETYL-COA CARBOXYLASE, LOW-FLOW ISCHEMIA, GLUCOSE-OXIDATION, MYOCARDIAL-METABOLISM, SUBSTRATE OXIDATION, CARDIAC EFFICIENCY, PERINATAL CHANGES, ENERGY SUBSTRATE, DICHLOROACETATE
  • Ankara Üniversitesi Adresli: Evet

Özet

The goal of this study was to investigate the effect of 1 mM exogenous lactate on cardiac function, and some metabolic parameters, such as glycolysis, glucose oxidation, lactate oxidation, and fatty acid oxidation, in isolated working rat hearts. Hearts from male Sprague-Dawley rats were isolated and perfused with 5 mM glucose, 1.2 mM palmitate, and 100 mu U/ml insulin with or without 1 mM lactate. The rates of glycolysis, glucose, lactate, and fatty acid oxidation were determined by supplementing the buffer with radiolabeled substrates. Cardiac function was similar between lactate+ and lactate- hearts. Glycolysis was not affected by 1 mM lactate. The addition of lactate did not alter glucose oxidation rates. Interestingly, palmitate oxidation rates almost doubled when 1 mM lactate was present in the perfusate. This study suggests that substrate supply to the heart is crucially important when evaluating the data from metabolic studies.