Akademik Veri Yönetim Sistemi
Journal of the Turkish Chemical Society, Section A: Chemistry, cilt.7, sa.2, ss.581-588, 2020 (Scopus)
© 2020, Turkish Chemical Society. All rights reserved.Ascorbic acid and selenium have potential use in the prevention of cancer. The role of antioxidant enzymes against cancer is to prevent oxidative damage. In this study, the effects of L-selenomethionine (234 µM), SeO2 (100 µM), ascorbic acid (0,313 mM, and 31,3 mM) on antioxidant enzyme activities in HepG2 cell line were studied. GPx, CAT, and SOD activities of the treated cells were measured spectrophotometrically. There was a significant increase in the activity of GPx in HepG2 cells treated with L-selenomethionine (234 µM), SeO2 (100 µM), and ascorbic acid (0,313 Mm and 31,3 mM). However, the activity of CAT was found significantly decreased in L-selenomethionine (234 µM), SeO2 (100 µM), ascorbic acid (31,3 mM) treated HepG2 cells. The SOD levels of HepG2 cells treated with ascorbic acid (0,313 mM and 31,3 mM) were found higher than the control cells. The results of the present study indicated that the antioxidant enzyme activities changed depending on L-selenomethionine, SeO2 application, and CAT activities changed depending on ascorbic acid doses.