Evaluation of hydrogen production by Clostridium strains on beet molasses

AVCI A., Kilic N., DÖNMEZ G., Donmez S.

ENVIRONMENTAL TECHNOLOGY, cilt.35, sa.3, ss.278-285, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 3
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1080/09593330.2013.826251
  • Dergi Adı: ENVIRONMENTAL TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.278-285
  • Anahtar Kelimeler: biohydrogen production, biofuel, Clostridium sp, molasses, BIOHYDROGEN PRODUCTION, FERMENTATION, BUTYRICUM, ACETOBUTYLICUM, SUBSTRATE, WATER, BEIJERINCKII, CONVERSION, WASTES, DNA
  • Ankara Üniversitesi Adresli: Evet

Özet

Clostridium acetobutylicum DSM 792, C. acetobutylicum DSM 1731 and two newly isolated bacteria defined as the members of genus Clostridium - based on the 16S rRNA analysis and biochemical traits - were characterized with regard to their hydrogen production in media containing increasing beet molasses concentrations. The highest hydrogen yield was observed for C. acetobutylicum DSM 792 with a yield of 2.8 mol H-2 mol(-1) hexose in medium including 60 g L-1 molasses. This bacterium also produced the maximum amount of hydrogen (5908.8 mL L-1) at the same molasses concentration. A slightly lower hydrogen yield was measured for C. acetobutylicum DSM 1731 (2.5 mol H-2 mol(-1) hexose) when grown on 40 g L-1 molasses. The new isolates Clostridium roseum C and Clostridium saccharoperbutylacetonicum PF produced hydrogen with yields of 2.0 mol H-2 mol(-1) hexose at 40 and 60 g L-1 molasses and 2.1 mol H-2 mol(-1) hexose at 40 g L-1 molasses, respectively.