Akademik Veri Yönetim Sistemi
Ataturk Universitesi Veteriner Bilimleri Dergisi, cilt.15, sa.1, ss.76-83, 2020 (Scopus)
© 2020 Ataturk Universitesi. All rights reserved.Quality analysis of semen has started to gain value in the recent scientific world in terms of defining infertility and assisted reproductive techniques. Flow cytometry, the most advanced method of choice in these analyzes, is a method based on measuring the quality and quantity of particles including cells, nucleus, chromosomal structures and various particles with its sizes and structures in a flowing suspension by means of lasers and detectors. For this purpose, the particles that are stained with fluorochrome molecules are exposed to the laser beam scattered from the optical system after being unified, and the returning rays are collected and converted into electronic signals. Electronic signals are evaluated with softwares. Within a wide range of uses, i.e. the concentration of spermatozoa; plasma membrane and acrosome integrity; apoptosis; mitochondrial membrane potential, capacitation status; oxidative stress; lipid peroxidation; DNA integrity and gender determination purposes etc., it has been used as infertility diagnostic methods and assisted reproductive techniques which have been developed, firstly in human andrology laboratories and then in veterinary andrology laboratories. The aim of this review is to explain the role and importance of flow cytometry and in determining sperm quality and to provide detailed information about the relevant protocols.