A novel and ultrasensitive sandwich-type electrochemical immunosensor based on delaminated MXene@AuNPs as signal amplification for prostate specific antigen (PSA) detection and immunosensor validation

Medetalibeyoglu H., Kotan G., Atar N., Yola M. L.

TALANTA, cilt.220, 2020 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 220
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.talanta.2020.121403
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, L'Année philologique, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, Linguistic Bibliography, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Ankara Üniversitesi Adresli: Hayır

Özet

In this study, an ultra-sensitive electrochemical immunosensor for PSA detection was designed. Firstly, gold nanoparticles (AuNPs)/p-aminothiophenol (ATP) functionalized graphene oxide (GO) composite (AuNPs-ATPGO) was constructed and modified to glassy carbon electrode (AuNPs-ATPGO/GCE). AuNPs-ATPGO/GCE was utilized as immunosensor platform to increase the amount of PSA antibody1 (Ab(1)). After that, selfassembled delaminated MXene-gold nanoparticles (d-Ti3C2TX MXene@AuNPs) was used to label PSA seconder antibody2 (Ab(2)) as signal amplification. The prepared AuNPs-ATPGO, d-Ti3C2TX MXene@AuNPs and electrochemical immunosensor was charecterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscope (SEM), x-ray diffraction (XRD) method, x-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR). The linearity range and detection limit (LOD) were obtained as 0.01-1.0 pg mL(-1) and 3.0 fg mL(-1), respectively.