Akademik Veri Yönetim Sistemi
Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.47, sa.2, ss.1-8, 2023 (Scopus)
Objective: Colorectal cancer is a rapidly increasing disease worldwide, and almost half of the diagnosed patients die from this disease each year. The methods used in the treatment of colorectal cancer, including traditional treatment methods such as surgery, radiotherapy and current chemotherapy options, have low effectiveness and have many side effects. Because of all these problems, the importance of developing new agents for the treatment of colorectal cancer is increasing. Orsinol is a secondary metabolite isolated from lichens, and there are findings regarding the antioxidant, antimicrobial and antidepressant activity of the compound. In recent years, research on the anticancer activity of the compound has also started to take place in the literature. In this study, it was aimed to investigate the efficacy of orcinol on cell proliferation and apoptosis in human SW480 colorectal cancer cells. Material and Method: Within the scope of the study, SW480 human colorectal cancer cells were used and cultured in DMEM medium. Orcinol was dissolved with dimethylsulfoxide to prepare a stock solution and applied to the cells in a concentration range of 1-25 mM. The effect of orcinol on cell viability was determined by MTT test. The apoptotic activity of the compound was evaluated with Annexin V binding assay using the Muse Cell Analyzer. Result and Discussion: The results of MTT analysis showed that orcinol significantly decreased cell viability at 5 mM and above (p<0.05). While cell viability was 100.00±6.14% in the control group, it was determined as 12.50±0.65% in cells treated with 25 mM orcinol (p<0.0001). According to Annexin V binding analysis findings, the early apoptotic cell population was 12.06±1.22% in the 25 mM orcinol treated group, while it was 0.60±0.11% in the control group. The findings obtained from the study showed that that orcinol has a cytotoxic effect at high concentration on SW480 colorectal cancer cells, and further studies are needed to increase the efficiency of the compound and to elucidate its mechanism of action.