Akademik Veri Yönetim Sistemi
FRESENIUS ENVIRONMENTAL BULLETIN, cilt.30, sa.7A, ss.9244-9249, 2021 (SCI-Expanded)
The priority of plant tissue culture studies is the development of an efficient regeneration protocol for the plant of interest. This study was aimed to develop an effective regeneration protocol for the important medicinal plant, bush basil (Ocimum minimum L.), from axillary meristem explants. In the study, seeds of Ocimum minimum L. were collected from experimental area of Department of Field Crops. Faculty of Agriculture. Ankara University. Seeds were placed in sterile bottles having 40% commercial bleach (5% sodium hypochlorite) and were shaken for 10 min at room temperature. This was followed by 3-4 washes with sterile distilled water. Sterilized seeds were germinated on a basal medium of Murashige and Skoog's (MS) mineral salts and vitamins, 3% sucrose, and 0.7% agar in Magenta vessels (15x15 cm). Axillary meristem explants were excised from 4-week-old sterile seedlings. For shoot regeneration, axillary meristem explants were cultured for 3 weeks on MS medium supplemented with different concentrations of BAP (2, 4 and 6 mg l(-1)) and 0.05 mg mg l(-1) NAA, All cultures were incubated under a cool white fluorescent light (27 mu mol m(-2) s(-1)) with a 16 h light/8 h dark photoperiod in a growth chamber at 25 +/- 1 degrees C. The pH of the medium was adjusted to 5.8 and autoclaved at 120 degrees C for 20 min Shoot regeneration percentage, shoot number per explant and total shoot number per Magenta vessel were recorded 3 weeks after culture initiation. Regenerated shoots were transferred to Magenta vessels (15x15 cm) containing 100 ml MS medium; they were incubated for two weeks at 25 +/- 1 degrees C to induce root formation. Rooted shoots were then transferred to pots in a growth room for two weeks where light. temperature and humidity were controlled. Humidity was decreased gradually from 100% to 40% during two weeks for acclimatization of seedlings. After two weeks, plantlets were moved to a greenhouse. The results showed that the highest values for observed parameters were obtained from MS basal medium containing 4 mg l(-1) BAP and 0.05 mg l(-1) NAA. As a result of this study. an effective, reliable and fast regeneration protocol has been developed for basil. an important medicinal plant for tissue culture studies.