LC method for the analysis of paracetamol, caffeine and codeine phosphate in pharmaceutical preparations

Kartal M.

Journal of Pharmaceutical and Biomedical Analysis, vol.26, no.5-6, pp.857-864, 2001 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 5-6
  • Publication Date: 2001
  • Doi Number: 10.1016/s0731-7085(01)00527-1
  • Journal Name: Journal of Pharmaceutical and Biomedical Analysis
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.857-864
  • Keywords: Acetaminophen, Caffeine, Codeine phosphate, High-performance liquid chromatography, Paracetamol, Validation
  • Ankara University Affiliated: Yes

Abstract

An accurate, simple, reproducible and sensitive method for the determination of paracetamol, caffeine and codeine phosphate has been developed and validated. Paracetamol, caffeine and codeine phosphate were separated using a μBondapack C8 column by isocratic elution with flow rate 1.0 ml/min. The mobile phase composition was 420/20/30/30 (v/v/v/v) 0.01 M KH2PO4, methanol, acetonitrile, isopropyl alcohol and spectrophotometric detection was carried out at 215 nm. The linear range of detection for paracetamol, caffeine and codeine phosphate were between 0.400 and 1500 μg/ml; 0.075 and 90 μg/ml; 0.300 and 30 μg/ml, respectively. The method has been shown to be linear, reproducible, specific, sensitive and rugged. © 2001 Elsevier Science B.V. All rights reserved.