Akademik Veri Yönetim Sistemi
35th Annual Northeast Bioengineering Conference, Cambridge, Kanada, 3 - 05 Nisan 2009, ss.366-367
We created 3D tissue constructs epitaxially by printing cell-laden hydrogel droplets. The ability to bioengineer 3D tissues is a powerful new approach to treat diverse diseases such as cancer, loss of tissue function, or organ failure. Inspired by the molecular beam epitaxy technique, a common semiconductor manufacturing technology, we present a platform that prints the first example of a 3D smooth muscle (SMC) patch (5mm x 5mm x 81 Am) consisting of multiple cell-laden hydrogel layers. The bioprinting platform that we developed allows (i) high throughput patterning of SMCs encapsulated in collagen hydrogel droplets (3 seconds/patch), (ii) microscale spatial and temporal droplet placement control (proximal axis: 18 +/- 15 mu m, distal axis:0 +/- 10 mu m), (iii) printing of 3D cell-laden hydrogel structures (16.2 mu m thick per layer), (iv) cell seeding uniformity (85 +/- 13 cells/mm(2) at 1 million cells/ml; 186 +/- 77 cells/mm(2) at 10 million cells/ml). This new platform to print 3D tissue constructs has potential for future therapeutic value in regenerative medicine and tissue engineering leading to printed replacement organs.