Adventitious shoot regeneration and micropropagation in <i>Calendula officinalis</i> L.

Cocu S., Uranbey S., Ipek A., Khawar K., Sarihan E., Kaya M., ...More

BIOLOGIA PLANTARUM, no.3, pp.449-451, 2004 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2004
  • Doi Number: 10.1023/b:biop.0000041102.79647.b6
  • Journal Name: BIOLOGIA PLANTARUM
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.449-451
  • Keywords: clonal propagation, cotyledon, cotyledonary node, hypocotyl, indole-3-butyric acid, kinetin, alpha-naphthalene-acetic acid, organogenesis, thidiazuron, THIDIAZURON
  • Ankara University Affiliated: Yes

Abstract

Hypocotyl, cotyledon and cotyledonary node explants of Calendula officinalis L were cultured on Murashige and Skoog ( MS) media supplemented with various concentrations of thidiazuron (TDZ), kinetin ( KIN), alpha-naphthaleneacetic acid (NAA) and indole-3-butyric acid ( IBA) to induce adventitious shoot regeneration and micropropagation. The highest frequency of adventitious shoot regeneration was achieved from hypocotyl and cotyledon explants on MS media supplemented with 0.75 mg dm(-3) TDZ and either 0.25 or 0.50 mg dm(-3) IBA. Efficient in vitro clonal propagation was also induced from cotyledonary nodes on a range of media supplemented with 0.75 mg dm(-3) TDZ and 0.05 mg dm(-3) NAA or 2 mg dm(-3) KIN and 1 mg dm(-3) NAA. Regenerated shoots were excised and rooted in MS medium supplemented with 1 mg dm(-3) NAA. The rooted plantlets were finally transferred to pots.