Myogenic Differentiation of ASCs Using Biochemical and Biophysical Induction

Huri P., Morrissette-McAlmon J., Grayson W. L.

ADIPOSE-DERIVED STEM CELLS: METHODS AND PROTOCOLS, 2ND EDITION, cilt.1773, ss.123-135, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1773
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/978-1-4939-7799-4_10
  • Dergi Adı: ADIPOSE-DERIVED STEM CELLS: METHODS AND PROTOCOLS, 2ND EDITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.123-135
  • Anahtar Kelimeler: Adipose-derived stem/stromal cell, Cyclic strain, Dynamic culture, Myogenesis, Skeletal muscle, 5-azacytidine, STEM-CELLS, SATELLITE CELLS, TISSUE, 5-AZACYTIDINE, PROMOTES
  • Ankara Üniversitesi Adresli: Evet

Özet

Adipose-derived stem/stromal cells (ASCs) constitute a very promising source for cell therapy and tissue engineering approaches as they can be easily obtained in large quantities with comparatively minimal patient discomfort. Moreover, ASCs have multilineage differentiation capacity. Among these, differentiation capacity along the myogenic lineage is of particular interest since myogenic precursors are scarce and obtaining a large number of cells from skeletal muscle biopsies is difficult. Here, we describe a method to effectively induce ASC myogenesis through the combination of biochemical (cocktail including 5-azacytidine and horse serum) and biophysical (dynamic culture via uniaxial cyclic strain) stimulation. This method results in multinucleated cells that are positive in myogenic markers including Pax 3/7, desmin, myoD, and myosin heavy chain.