A genotypic mutation technique: Restriction site mutation method

SÜZEN H. S., Parry J.

Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.28, sa.2, ss.85-97, 1999 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 2
  • Basım Tarihi: 1999
  • Dergi Adı: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.85-97
  • Anahtar Kelimeler: p53, PCR, ras, Restriction site mutation
  • Ankara Üniversitesi Adresli: Evet

Özet

Although cellular mutation analysis techniques are still used for the identification of mutagens, they are unsuitable for the study of disease-related mutations. Restriction site mutation (RSM) is an alternative method to traditional mutation recognition techniques that have some limitations. RSM technique aims of selecting certain DNA target by restriction enzyme digestion and resistant restriction enzyme recognition sequences (mutant) are amplified using polymerase chain reaction (PCR). In this study, RSM has been developed as a genotypic mutation method in the rat. The method has been applied to rat p53 and ras genes. Since RSM is the combination of restriction enzyme digestion and PCR, a number of factors have been investigated for use in the RSM. Several suitable restriction enzymes and primer pairs have been defined for analyzing base changes in the rat p53 and ras genes.